Detailed information of TA system TA025653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2324305..2324907
• Replicon: chromosome
• Accession: NC_016026
• Organism: Micavibrio aeruginosavorus ARL-13

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G2KT78
• Locus tag: MICA_RS11035
• Protein ID: WP_014103845.1
• Coordinates: 2324305..2324586 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MICA_RS11040
• Protein ID: WP_014103846.1
• Coordinates: 2324602..2324907 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MICA_RS11010	2319821..2320498	+	678	WP_014103840.1	HAD-IA family hydrolase	-
MICA_RS11015	2320553..2321293	-	741	WP_014103841.1	hypothetical protein	-
MICA_RS11020	2321536..2321775	+	240	WP_014103842.1	acyl carrier protein	-
MICA_RS11025	2321889..2323160	+	1272	WP_041794088.1	beta-ketoacyl-ACP synthase II	-
MICA_RS11030	2323172..2324209	+	1038	WP_014103844.1	endolytic transglycosylase MltG	-
MICA_RS11035	2324305..2324586	+	282	WP_014103845.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MICA_RS11040	2324602..2324907	+	306	WP_014103846.1	HigA family addiction module antidote protein	Antitoxin
MICA_RS11050	2325157..2325432	-	276	WP_014103847.1	hypothetical protein	-
MICA_RS11055	2325436..2325681	-	246	WP_014103848.1	hypothetical protein	-
MICA_RS11060	2325818..2326366	+	549	WP_014103849.1	prolyl-tRNA synthetase associated domain-containing protein	-
MICA_RS11065	2326372..2327325	+	954	WP_014103850.1	tetratricopeptide repeat protein	-
MICA_RS11070	2327588..2328271	+	684	WP_014103852.1	LON peptidase substrate-binding domain-containing protein	-
MICA_RS11075	2328304..2328489	+	186	WP_014103853.1	Trm112 family protein	-
MICA_RS11080	2328507..2329676	+	1170	WP_014103854.1	2-isopropylmalate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (10-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10809.42 Da
• Isoelectric Point: 7.8293

>T25653 WP_014103845.1 NC_016026:2324305-2324586 [Micavibrio aeruginosavorus ARL-13]
MIRSFACKDTAKIWEGLKVRGFPGDMQDRALMKLRLIDAAQTVDDLKIPPGNRLEDLRGDRKGQKSIRINDQWRICFVWK
DGDAFDVEITDYH

Nucleotide

• Length: 282 bp

>T25653 NC_016026:2324305-2324586 [Micavibrio aeruginosavorus ARL-13]
ATGATCCGGTCTTTTGCCTGTAAGGACACAGCAAAAATCTGGGAAGGCTTGAAAGTACGGGGCTTTCCGGGGGATATGCA
GGATCGGGCGTTGATGAAATTGCGCCTGATTGATGCGGCACAGACCGTGGACGACCTGAAAATTCCGCCGGGAAACCGGC
TGGAGGATTTAAGGGGTGACCGCAAAGGCCAAAAAAGCATTCGGATCAATGATCAGTGGCGCATATGCTTTGTTTGGAAA
GACGGCGATGCGTTTGATGTTGAAATCACAGATTACCATTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11258.99 Da
• Isoelectric Point: 9.0906

>AT25653 WP_014103846.1 NC_016026:2324602-2324907 [Micavibrio aeruginosavorus ARL-13]
MTTHLKNPHPGDILAVEFLEPLGLSQNALAKAIDVPANRINEIIRGRRGITADTDVRLARYFGLSEGFFLRLQNAYDLME
AHRAIPATELRRIKPHAQACA

Nucleotide

• Length: 306 bp

>AT25653 NC_016026:2324602-2324907 [Micavibrio aeruginosavorus ARL-13]
ATGACCACCCACCTTAAAAACCCTCACCCCGGCGATATTTTGGCCGTGGAGTTTTTGGAGCCGCTGGGCCTGTCGCAAAA
TGCGCTGGCCAAGGCGATTGACGTGCCCGCGAACCGTATCAATGAAATCATTCGCGGGCGGCGCGGCATTACGGCGGATA
CAGATGTCCGGCTGGCGCGGTATTTCGGCTTGTCCGAGGGGTTTTTCCTGCGCCTGCAAAACGCCTATGACCTGATGGAG
GCGCACCGCGCCATTCCCGCCACGGAACTGCGCCGGATTAAACCACACGCGCAGGCCTGTGCCTGA

Structures

Toxin

• AlphaFold DB: G2KT78