Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3859915..3860456 Replicon chromosome
Accession NZ_CP103745
Organism Escherichia coli strain 3090

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag M5T02_RS18750 Protein ID WP_000615983.1
Coordinates 3860178..3860456 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag M5T02_RS18745 Protein ID WP_000729703.1
Coordinates 3859915..3860175 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T02_RS18725 (3855590) 3855590..3856375 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5T02_RS18730 (3856347) 3856347..3858059 + 1713 Protein_3666 flagellar biosynthesis protein FlhA -
M5T02_RS18735 (3858283) 3858283..3858780 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
M5T02_RS18740 (3858956) 3858956..3859705 - 750 WP_000056849.1 C40 family peptidase -
M5T02_RS18745 (3859915) 3859915..3860175 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M5T02_RS18750 (3860178) 3860178..3860456 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M5T02_RS18755 (3860612) 3860612..3861352 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M5T02_RS18760 (3861323) 3861323..3862090 - 768 WP_000333380.1 class II glutamine amidotransferase -
M5T02_RS18765 (3862296) 3862296..3862874 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T256489 WP_000615983.1 NZ_CP103745:3860178-3860456 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT256489 WP_000729703.1 NZ_CP103745:3859915-3860175 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References