Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3853718..3854412 Replicon chromosome
Accession NZ_CP103745
Organism Escherichia coli strain 3090

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M5T02_RS18710 Protein ID WP_001263489.1
Coordinates 3853718..3854116 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M5T02_RS18715 Protein ID WP_000554758.1
Coordinates 3854119..3854412 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3849306) 3849306..3849386 - 81 NuclAT_11 - -
- (3849306) 3849306..3849386 - 81 NuclAT_11 - -
- (3849306) 3849306..3849386 - 81 NuclAT_11 - -
- (3849306) 3849306..3849386 - 81 NuclAT_11 - -
M5T02_RS18685 (3849982) 3849982..3850440 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M5T02_RS18690 (3850701) 3850701..3852158 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M5T02_RS18695 (3852215) 3852215..3852736 - 522 Protein_3659 peptide chain release factor H -
M5T02_RS18700 (3852732) 3852732..3852938 - 207 Protein_3660 RtcB family protein -
M5T02_RS18705 (3853256) 3853256..3853708 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M5T02_RS18710 (3853718) 3853718..3854116 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5T02_RS18715 (3854119) 3854119..3854412 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5T02_RS18720 (3854464) 3854464..3855519 - 1056 WP_001226164.1 DNA polymerase IV -
M5T02_RS18725 (3855590) 3855590..3856375 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5T02_RS18730 (3856347) 3856347..3858059 + 1713 Protein_3666 flagellar biosynthesis protein FlhA -
M5T02_RS18735 (3858283) 3858283..3858780 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T256488 WP_001263489.1 NZ_CP103745:c3854116-3853718 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT256488 WP_000554758.1 NZ_CP103745:c3854412-3854119 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References