Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1845217..1845719 Replicon chromosome
Accession NZ_CP103745
Organism Escherichia coli strain 3090

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag M5T02_RS08765 Protein ID WP_000767831.1
Coordinates 1845465..1845719 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag M5T02_RS08760 Protein ID WP_001259255.1
Coordinates 1845217..1845468 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T02_RS08735 (1840394) 1840394..1841461 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
M5T02_RS08740 (1841461) 1841461..1842531 - 1071 WP_000108939.1 histidinol-phosphate transaminase -
M5T02_RS08745 (1842528) 1842528..1843832 - 1305 WP_001375298.1 histidinol dehydrogenase -
M5T02_RS08750 (1843838) 1843838..1844737 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
M5T02_RS08755 (1844883) 1844883..1844933 - 51 WP_001364200.1 his operon leader peptide -
M5T02_RS08760 (1845217) 1845217..1845468 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
M5T02_RS08765 (1845465) 1845465..1845719 + 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
M5T02_RS08770 (1845802) 1845802..1846626 + 825 WP_000754737.1 SDR family oxidoreductase -
M5T02_RS08775 (1846672) 1846672..1847601 + 930 WP_048217808.1 LysR substrate-binding domain-containing protein -
M5T02_RS08780 (1847816) 1847816..1847878 + 63 WP_010723108.1 membrane protein YoeI -
M5T02_RS08785 (1847868) 1847868..1849226 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
M5T02_RS08790 (1849405) 1849405..1850463 + 1059 WP_048217810.1 thiosulfate utilization transporter TsuA/YeeE -
M5T02_RS08795 (1850477) 1850477..1850704 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T256478 WP_000767831.1 NZ_CP103745:1845465-1845719 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT256478 WP_001259255.1 NZ_CP103745:1845217-1845468 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References