Detailed information of TA system TA025646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4904140..4904687
• Replicon: chromosome
• Accession: NC_016010
• Organism: Xanthomonas axonopodis pv. citrumelo F1 (Xanthomonas alfalfae)

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: XACM_RS22680
• Protein ID: WP_082244529.1
• Coordinates: 4904448..4904687 (-)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: XACM_RS20770
• Protein ID: WP_008571772.1
• Coordinates: 4904140..4904430 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XACM_RS20750	4899447..4899779	+	333	WP_003482333.1	hypothetical protein	-
XACM_RS20755	4900113..4902038	+	1926	WP_014091549.1	M61 family metallopeptidase	-
XACM_RS23490	4902106..4902285	+	180	WP_003482327.1	hypothetical protein	-
XACM_RS20765	4902388..4903782	-	1395	WP_014091550.1	nucleoside hydrolase	-
XACM_RS20770	4904140..4904430	-	291	WP_008571772.1	HigA family addiction module antidote protein	Antitoxin
XACM_RS22680	4904448..4904687	-	240	WP_082244529.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XACM_RS20780	4904785..4907160	-	2376	WP_014091552.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-79)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 9339.76 Da
• Isoelectric Point: 10.5666

>T25646 WP_082244529.1 NC_016010:c4904687-4904448 [Xanthomonas axonopodis pv. citrumelo F1]
MGERSRRLPADIQLVARRTLRMLNAAAHLDNLPIPPANRLEASKGERRGQYRIRINDQWRICFRWMEGDVVQVEIVDYH

Nucleotide

• Length: 240 bp

>T25646 NC_016010:c4904687-4904448 [Xanthomonas axonopodis pv. citrumelo F1]
CTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCAATTGGTCGCGCGCCGCACGTTGCGCATGCTCAATGCCGCCGC
GCATCTCGACAATCTGCCGATTCCGCCCGCCAACCGGCTGGAAGCGTCGAAGGGCGAGCGGCGCGGCCAGTACCGCATCC
GGATCAACGATCAATGGCGCATCTGCTTCCGATGGATGGAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10337.97 Da
• Isoelectric Point: 6.4665

>AT25646 WP_008571772.1 NC_016010:c4904430-4904140 [Xanthomonas axonopodis pv. citrumelo F1]
MTVLPNIHPGEILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERVWLGLQADDELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT25646 NC_016010:c4904430-4904140 [Xanthomonas axonopodis pv. citrumelo F1]
ATGACGGTCCTACCCAATATCCACCCTGGCGAAATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGTCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGAACCTCCGAGCGCGTCTGGCTTGGCCTGCAGGCCGACGACGAACTGGAGCAGGCG
CATCGCGCGCTGGGCGACTTGCCGGCGCGGATCAAGCGGCTGGCGGCATGA