Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3518421..3519100 Replicon chromosome
Accession NZ_CP103742
Organism Escherichia coli O25b:H4-ST131 strain 2822

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag M5R22_RS17290 Protein ID WP_000057523.1
Coordinates 3518798..3519100 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag M5R22_RS17285 Protein ID WP_000806442.1
Coordinates 3518421..3518762 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R22_RS17275 (3514665) 3514665..3515597 - 933 WP_000883041.1 glutaminase A -
M5R22_RS17280 (3515859) 3515859..3518363 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
M5R22_RS17285 (3518421) 3518421..3518762 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
M5R22_RS17290 (3518798) 3518798..3519100 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5R22_RS17295 (3519233) 3519233..3520027 + 795 WP_000365147.1 TraB/GumN family protein -
M5R22_RS17300 (3520231) 3520231..3520710 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
M5R22_RS17305 (3520734) 3520734..3521534 + 801 WP_000439798.1 hypothetical protein -
M5R22_RS17310 (3521531) 3521531..3522034 + 504 WP_000667000.1 hypothetical protein -
M5R22_RS17315 (3522072) 3522072..3523724 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T256459 WP_000057523.1 NZ_CP103742:c3519100-3518798 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT256459 WP_000806442.1 NZ_CP103742:c3518762-3518421 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References