Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 642568..643295 Replicon chromosome
Accession NZ_CP103742
Organism Escherichia coli O25b:H4-ST131 strain 2822

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag M5R22_RS03180 Protein ID WP_000550189.1
Coordinates 642568..642882 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5R22_RS03185 Protein ID WP_000560269.1
Coordinates 642879..643295 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R22_RS03160 (638725) 638725..639711 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
M5R22_RS03165 (639790) 639790..640482 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
M5R22_RS03170 (640559) 640559..641062 - 504 WP_001295542.1 M48 family metallopeptidase -
M5R22_RS03175 (641147) 641147..642283 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
M5R22_RS03180 (642568) 642568..642882 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
M5R22_RS03185 (642879) 642879..643295 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
M5R22_RS03190 (643340) 643340..645358 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
M5R22_RS03195 (645584) 645584..647935 - 2352 WP_000695431.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T256448 WP_000550189.1 NZ_CP103742:642568-642882 [Escherichia coli O25b:H4-ST131]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT256448 WP_000560269.1 NZ_CP103742:642879-643295 [Escherichia coli O25b:H4-ST131]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References