Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 602041..602840 Replicon chromosome
Accession NZ_CP103742
Organism Escherichia coli O25b:H4-ST131 strain 2822

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag M5R22_RS02960 Protein ID WP_000347251.1
Coordinates 602041..602505 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag M5R22_RS02965 Protein ID WP_001296435.1
Coordinates 602505..602840 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R22_RS02930 (597042) 597042..597476 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5R22_RS02935 (597494) 597494..598372 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5R22_RS02940 (598362) 598362..599141 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5R22_RS02945 (599152) 599152..599625 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5R22_RS02950 (599648) 599648..600928 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5R22_RS02955 (601177) 601177..601986 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5R22_RS02960 (602041) 602041..602505 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5R22_RS02965 (602505) 602505..602840 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5R22_RS02970 (602989) 602989..604560 - 1572 WP_001273763.1 galactarate dehydratase -
M5R22_RS02975 (604935) 604935..606269 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
M5R22_RS02980 (606285) 606285..607055 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T256447 WP_000347251.1 NZ_CP103742:c602505-602041 [Escherichia coli O25b:H4-ST131]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT256447 WP_001296435.1 NZ_CP103742:c602840-602505 [Escherichia coli O25b:H4-ST131]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References