Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1122913..1123454 Replicon chromosome
Accession NZ_CP103739
Organism Escherichia coli strain 3359

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag M5T33_RS05445 Protein ID WP_000615976.1
Coordinates 1122913..1123191 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag M5T33_RS05450 Protein ID WP_000729704.1
Coordinates 1123194..1123454 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T33_RS05430 (1120495) 1120495..1121073 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
M5T33_RS05435 (1121279) 1121279..1122046 + 768 WP_001365365.1 class II glutamine amidotransferase -
M5T33_RS05440 (1122017) 1122017..1122757 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M5T33_RS05445 (1122913) 1122913..1123191 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M5T33_RS05450 (1123194) 1123194..1123454 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M5T33_RS05460 (1124497) 1124497..1125270 + 774 WP_016245244.1 C40 family peptidase -
M5T33_RS05465 (1125446) 1125446..1125943 + 498 WP_000006242.1 REP-associated tyrosine transposase RayT -
M5T33_RS05470 (1126167) 1126167..1127879 - 1713 Protein_1054 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1123645..1124148 503
- inside Prophage - IlpA / gmhA/lpcA / yagV/ecpE / yagW/ecpD / yagX/ecpC / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR 1082397..1170984 88587


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T256424 WP_000615976.1 NZ_CP103739:c1123191-1122913 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT256424 WP_000729704.1 NZ_CP103739:c1123454-1123194 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References