Detailed information of TA system TA025641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3149504..3150189
• Replicon: chromosome
• Accession: NC_015976
• Organism: Sphingobium sp. SYK-6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G2IL31
• Locus tag: SLG_RS14395
• Protein ID: WP_014077222.1
• Coordinates: 3149504..3149866 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SLG_RS14400
• Protein ID: WP_197535709.1
• Coordinates: 3149893..3150189 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SLG_RS14370	3145573..3146991	+	1419	WP_014077219.1	DEAD/DEAH box helicase	-
SLG_RS14385	3147498..3148730	+	1233	WP_014077220.1	tyrosine-type recombinase/integrase	-
SLG_RS14390	3148743..3148946	-	204	WP_014077221.1	AlpA family phage regulatory protein	-
SLG_RS14395	3149504..3149866	+	363	WP_014077222.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SLG_RS14400	3149893..3150189	+	297	WP_197535709.1	XRE family transcriptional regulator	Antitoxin
SLG_RS14405	3150180..3150425	-	246	WP_041392071.1	conjugal transfer protein TraD	-
SLG_RS14410	3150463..3150768	-	306	WP_014077225.1	conjugal transfer protein TraD	-
SLG_RS14415	3150943..3153969	+	3027	WP_014077226.1	Ti-type conjugative transfer relaxase TraA	-
SLG_RS14420	3153987..3154745	+	759	WP_014077227.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3147318..3164703	17385

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (3-81)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13293.11 Da
• Isoelectric Point: 6.4865

>T25641 WP_014077222.1 NC_015976:3149504-3149866 [Sphingobium sp. SYK-6]
MPWTVRNHDAFDAEYDALPDAVQDELLAATGLLELYGPRLGRPHADTLGGSRHANMKELRFNADGGVWRVAFAFDPERQA
ILLVAGDKAGVAQKRFYKTLIAMADKRFDEHLAALAAKEK

Nucleotide

• Length: 363 bp

>T25641 NC_015976:3149504-3149866 [Sphingobium sp. SYK-6]
ATGCCGTGGACGGTTCGCAACCATGATGCCTTTGATGCCGAATATGACGCGTTGCCCGATGCGGTGCAGGACGAGCTATT
GGCGGCAACCGGCCTATTGGAACTTTATGGCCCCCGGCTCGGTCGCCCCCATGCCGATACGCTTGGCGGATCACGGCACG
CGAACATGAAGGAATTGCGCTTCAATGCCGATGGCGGCGTTTGGCGGGTGGCCTTCGCGTTCGACCCGGAGCGGCAAGCG
ATCCTGCTGGTTGCGGGCGACAAGGCAGGTGTGGCGCAGAAGCGGTTTTACAAGACGTTGATCGCCATGGCCGACAAGCG
GTTTGACGAGCATCTGGCGGCGCTGGCCGCGAAGGAGAAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10932.60 Da
• Isoelectric Point: 8.6973

>AT25641 WP_197535709.1 NC_015976:3149893-3150189 [Sphingobium sp. SYK-6]
MAALPEERRRRIEARAQELVAEVEGLKALRQLAERSQEQIAETLGVKQPSVHKIERQTDLYLSTLRRFVEAAGGKLELRV
ELPGKGVLHLTGVGDLHA

Nucleotide

• Length: 297 bp

>AT25641 NC_015976:3149893-3150189 [Sphingobium sp. SYK-6]
ATGGCCGCCCTGCCCGAAGAGCGCCGCCGCCGCATCGAGGCGCGCGCGCAGGAATTGGTTGCGGAGGTCGAGGGACTGAA
AGCGTTGCGCCAGCTTGCCGAGCGCAGCCAAGAGCAGATTGCAGAAACCTTGGGCGTGAAGCAGCCTTCCGTTCACAAGA
TCGAGCGGCAGACCGACCTTTACCTTTCGACGCTGCGCCGGTTCGTGGAGGCAGCGGGCGGCAAGCTGGAACTTCGCGTC
GAGTTGCCGGGCAAGGGCGTGCTGCATCTGACAGGCGTTGGCGATCTTCACGCCTGA

Structures

Toxin

• AlphaFold DB: G2IL31