Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4925444..4926046 Replicon chromosome
Accession NZ_CP103718
Organism Escherichia coli strain 1579

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag M5S87_RS23730 Protein ID WP_000897305.1
Coordinates 4925735..4926046 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5S87_RS23725 Protein ID WP_000356395.1
Coordinates 4925444..4925734 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S87_RS23690 (4921067) 4921067..4921969 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
M5S87_RS23695 (4921966) 4921966..4922601 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
M5S87_RS23700 (4922598) 4922598..4923527 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
M5S87_RS23705 (4923709) 4923709..4923951 - 243 WP_021523315.1 hypothetical protein -
M5S87_RS23710 (4924170) 4924170..4924388 - 219 WP_001295676.1 CopG family transcriptional regulator -
M5S87_RS23715 (4924807) 4924807..4925085 - 279 WP_001306650.1 hypothetical protein -
M5S87_RS23720 (4925147) 4925147..4925359 - 213 WP_000197769.1 hypothetical protein -
M5S87_RS23725 (4925444) 4925444..4925734 - 291 WP_000356395.1 NadS family protein Antitoxin
M5S87_RS23730 (4925735) 4925735..4926046 - 312 WP_000897305.1 hypothetical protein Toxin
M5S87_RS23735 (4926275) 4926275..4927183 + 909 WP_001306651.1 alpha/beta hydrolase -
M5S87_RS23740 (4927352) 4927352..4928266 - 915 WP_077634137.1 transposase -
M5S87_RS23745 (4928279) 4928279..4929166 - 888 Protein_4641 hypothetical protein -
M5S87_RS23750 (4929582) 4929582..4930523 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
M5S87_RS23755 (4930568) 4930568..4931005 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T256346 WP_000897305.1 NZ_CP103718:c4926046-4925735 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT256346 WP_000356395.1 NZ_CP103718:c4925734-4925444 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References