Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3516725..3517404 Replicon chromosome
Accession NZ_CP103710
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag M5S69_RS17475 Protein ID WP_000057523.1
Coordinates 3517102..3517404 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag M5S69_RS17470 Protein ID WP_000806442.1
Coordinates 3516725..3517066 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S69_RS17460 (3512969) 3512969..3513901 - 933 WP_000883041.1 glutaminase A -
M5S69_RS17465 (3514163) 3514163..3516667 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
M5S69_RS17470 (3516725) 3516725..3517066 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
M5S69_RS17475 (3517102) 3517102..3517404 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5S69_RS17480 (3517537) 3517537..3518331 + 795 WP_000365147.1 TraB/GumN family protein -
M5S69_RS17485 (3518535) 3518535..3519014 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
M5S69_RS17490 (3519182) 3519182..3519838 + 657 WP_015674862.1 hypothetical protein -
M5S69_RS17495 (3519835) 3519835..3520338 + 504 WP_000667000.1 hypothetical protein -
M5S69_RS17500 (3520376) 3520376..3522028 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T256286 WP_000057523.1 NZ_CP103710:c3517404-3517102 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT256286 WP_000806442.1 NZ_CP103710:c3517066-3516725 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References