Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3788321..3788862 Replicon chromosome
Accession NZ_CP103704
Organism Escherichia coli strain ST361

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag M5T41_RS18485 Protein ID WP_000615979.1
Coordinates 3788584..3788862 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag M5T41_RS18480 Protein ID WP_000729704.1
Coordinates 3788321..3788581 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T41_RS18460 (3783996) 3783996..3784781 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
M5T41_RS18465 (3784753) 3784753..3786465 + 1713 Protein_3617 flagellar biosynthesis protein FlhA -
M5T41_RS18470 (3786689) 3786689..3787186 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
M5T41_RS18475 (3787362) 3787362..3788135 - 774 WP_123297815.1 C40 family peptidase -
M5T41_RS18480 (3788321) 3788321..3788581 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M5T41_RS18485 (3788584) 3788584..3788862 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M5T41_RS18490 (3789018) 3789018..3789758 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M5T41_RS18495 (3789729) 3789729..3790496 - 768 WP_000333379.1 class II glutamine amidotransferase -
M5T41_RS18500 (3790702) 3790702..3791280 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T256264 WP_000615979.1 NZ_CP103704:3788584-3788862 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT256264 WP_000729704.1 NZ_CP103704:3788321-3788581 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References