Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3782124..3782818 Replicon chromosome
Accession NZ_CP103704
Organism Escherichia coli strain ST361

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag M5T41_RS18445 Protein ID WP_001263493.1
Coordinates 3782124..3782522 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag M5T41_RS18450 Protein ID WP_000554757.1
Coordinates 3782525..3782818 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3777784) 3777784..3777864 - 81 NuclAT_11 - -
- (3777784) 3777784..3777864 - 81 NuclAT_11 - -
- (3777784) 3777784..3777864 - 81 NuclAT_11 - -
- (3777784) 3777784..3777864 - 81 NuclAT_11 - -
M5T41_RS18415 (3777124) 3777124..3778368 - 1245 WP_000189541.1 esterase FrsA -
M5T41_RS18420 (3778460) 3778460..3778918 - 459 WP_061349133.1 xanthine phosphoribosyltransferase -
M5T41_RS18425 (3779179) 3779179..3780636 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
M5T41_RS18430 (3780693) 3780693..3781214 - 522 Protein_3610 peptide chain release factor H -
M5T41_RS18435 (3781213) 3781213..3781416 - 204 Protein_3611 RtcB family protein -
M5T41_RS18440 (3781662) 3781662..3782114 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
M5T41_RS18445 (3782124) 3782124..3782522 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5T41_RS18450 (3782525) 3782525..3782818 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5T41_RS18455 (3782870) 3782870..3783925 - 1056 WP_001226164.1 DNA polymerase IV -
M5T41_RS18460 (3783996) 3783996..3784781 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
M5T41_RS18465 (3784753) 3784753..3786465 + 1713 Protein_3617 flagellar biosynthesis protein FlhA -
M5T41_RS18470 (3786689) 3786689..3787186 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T256263 WP_001263493.1 NZ_CP103704:c3782522-3782124 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT256263 WP_000554757.1 NZ_CP103704:c3782818-3782525 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References