256211

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4182628..4183125 Replicon chromosome
Accession NZ_CP103694
Organism Escherichia coli strain ST12468

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5T31_RS20500 Protein ID WP_001173343.1
Coordinates 4182628..4182801 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M5T31_RS20505 Protein ID WP_000398619.1
Coordinates 4182829..4183125 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T31_RS20490 (4179298) 4179298..4180755 + 1458 WP_058905731.1 dipeptide/tripeptide permease DtpC -
M5T31_RS20495 (4180992) 4180992..4182509 + 1518 WP_001295074.1 lysine--tRNA ligase -
M5T31_RS20500 (4182628) 4182628..4182801 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5T31_RS20505 (4182829) 4182829..4183125 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5T31_RS20510 (4183352) 4183352..4183624 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M5T31_RS20515 (4183636) 4183636..4183866 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
M5T31_RS20520 (4184047) 4184047..4185678 + 1632 WP_001565376.1 sensor histidine kinase -
M5T31_RS20525 (4185675) 4185675..4186394 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4173774..4183125 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T256211 WP_001173343.1 NZ_CP103694:c4182801-4182628 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT256211 WP_000398619.1 NZ_CP103694:c4183125-4182829 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References