Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3633086..3633627 Replicon chromosome
Accession NZ_CP103694
Organism Escherichia coli strain ST12468

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag M5T31_RS17885 Protein ID WP_000615976.1
Coordinates 3633349..3633627 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag M5T31_RS17880 Protein ID WP_000729704.1
Coordinates 3633086..3633346 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T31_RS17860 (3628762) 3628762..3629547 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
M5T31_RS17865 (3629492) 3629492..3631230 + 1739 Protein_3499 flagellar biosynthesis protein FlhA -
M5T31_RS17870 (3631454) 3631454..3631951 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
M5T31_RS17875 (3632127) 3632127..3632900 - 774 WP_015675129.1 C40 family peptidase -
M5T31_RS17880 (3633086) 3633086..3633346 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M5T31_RS17885 (3633349) 3633349..3633627 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M5T31_RS17890 (3633783) 3633783..3634523 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M5T31_RS17895 (3634494) 3634494..3635261 - 768 WP_000333379.1 class II glutamine amidotransferase -
M5T31_RS17900 (3635467) 3635467..3636045 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3625928..3647045 21117


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T256209 WP_000615976.1 NZ_CP103694:3633349-3633627 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT256209 WP_000729704.1 NZ_CP103694:3633086-3633346 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References