256208

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3626890..3627584 Replicon chromosome
Accession NZ_CP103694
Organism Escherichia coli strain ST12468

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag M5T31_RS17845 Protein ID WP_001263493.1
Coordinates 3626890..3627288 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag M5T31_RS17850 Protein ID WP_000554757.1
Coordinates 3627291..3627584 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3622550) 3622550..3622630 - 81 NuclAT_9 - -
- (3622550) 3622550..3622630 - 81 NuclAT_9 - -
- (3622550) 3622550..3622630 - 81 NuclAT_9 - -
- (3622550) 3622550..3622630 - 81 NuclAT_9 - -
M5T31_RS17815 (3621890) 3621890..3623134 - 1245 WP_000189541.1 esterase FrsA -
M5T31_RS17820 (3623226) 3623226..3623684 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M5T31_RS17825 (3623945) 3623945..3625402 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
M5T31_RS17830 (3625459) 3625459..3625980 - 522 Protein_3492 peptide chain release factor H -
M5T31_RS17835 (3625979) 3625979..3626182 - 204 Protein_3493 RtcB family protein -
M5T31_RS17840 (3626428) 3626428..3626880 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
M5T31_RS17845 (3626890) 3626890..3627288 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5T31_RS17850 (3627291) 3627291..3627584 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5T31_RS17855 (3627636) 3627636..3628691 - 1056 WP_001226164.1 DNA polymerase IV -
M5T31_RS17860 (3628762) 3628762..3629547 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
M5T31_RS17865 (3629492) 3629492..3631230 + 1739 Protein_3499 flagellar biosynthesis protein FlhA -
M5T31_RS17870 (3631454) 3631454..3631951 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3625928..3647045 21117


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T256208 WP_001263493.1 NZ_CP103694:c3627288-3626890 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT256208 WP_000554757.1 NZ_CP103694:c3627584-3627291 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References