Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2460795..2461484 Replicon chromosome
Accession NZ_CP103687
Organism Klebsiella pneumoniae strain ST307

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag M5S48_RS12185 Protein ID WP_021469727.1
Coordinates 2460795..2461112 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag M5S48_RS12190 Protein ID WP_020804705.1
Coordinates 2461188..2461484 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S48_RS12155 (2456497) 2456497..2457006 + 510 WP_020802144.1 GNAT family N-acetyltransferase -
M5S48_RS12160 (2457016) 2457016..2457942 + 927 WP_032435210.1 amino acid ABC transporter permease -
M5S48_RS12165 (2457926) 2457926..2458705 + 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
M5S48_RS12170 (2458744) 2458744..2459595 + 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
M5S48_RS12175 (2459673) 2459673..2460290 + 618 WP_032425015.1 glutathione S-transferase family protein -
M5S48_RS12180 (2460361) 2460361..2460588 + 228 WP_002906690.1 tautomerase PptA -
M5S48_RS12185 (2460795) 2460795..2461112 + 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5S48_RS12190 (2461188) 2461188..2461484 + 297 WP_020804705.1 NadS family protein Antitoxin
M5S48_RS12195 (2461563) 2461563..2462009 + 447 WP_032435212.1 hypothetical protein -
M5S48_RS12200 (2462050) 2462050..2463666 - 1617 WP_004175961.1 carbohydrate porin -
M5S48_RS12205 (2463710) 2463710..2465092 - 1383 WP_004151222.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T256186 WP_021469727.1 NZ_CP103687:2460795-2461112 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT256186 WP_020804705.1 NZ_CP103687:2461188-2461484 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References