Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3523765..3524440 Replicon chromosome
Accession NZ_CP103679
Organism Enterobacter hormaechei strain MB4528 Variant

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag M5T06_RS16730 Protein ID WP_015571638.1
Coordinates 3523765..3524064 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag M5T06_RS16735 Protein ID WP_015571639.1
Coordinates 3524075..3524440 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T06_RS16720 (M5T06_16720) 3520142..3522334 + 2193 WP_017692801.1 type I secretion system permease/ATPase -
M5T06_RS16725 (M5T06_16725) 3522315..3523514 + 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
M5T06_RS16730 (M5T06_16730) 3523765..3524064 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5T06_RS16735 (M5T06_16735) 3524075..3524440 + 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
M5T06_RS16740 (M5T06_16740) 3524467..3525648 - 1182 WP_017382758.1 PLP-dependent aminotransferase family protein -
M5T06_RS16745 (M5T06_16745) 3525669..3526556 - 888 WP_033487545.1 LysR family transcriptional regulator -
M5T06_RS16750 (M5T06_16750) 3526654..3527256 + 603 WP_033487546.1 short chain dehydrogenase -
M5T06_RS16755 (M5T06_16755) 3527253..3527984 - 732 WP_017382760.1 methyltransferase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T256153 WP_015571638.1 NZ_CP103679:3523765-3524064 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT256153 WP_015571639.1 NZ_CP103679:3524075-3524440 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References