Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3568409..3569084 Replicon chromosome
Accession NZ_CP103674
Organism Enterobacter hormaechei strain 1074

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag M5T14_RS16925 Protein ID WP_015571638.1
Coordinates 3568409..3568708 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag M5T14_RS16930 Protein ID WP_015571639.1
Coordinates 3568719..3569084 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T14_RS16915 (M5T14_16915) 3564786..3566978 + 2193 WP_032654716.1 type I secretion system permease/ATPase -
M5T14_RS16920 (M5T14_16920) 3566959..3568158 + 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
M5T14_RS16925 (M5T14_16925) 3568409..3568708 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5T14_RS16930 (M5T14_16930) 3568719..3569084 + 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
M5T14_RS16935 (M5T14_16935) 3569111..3570292 - 1182 WP_017382758.1 PLP-dependent aminotransferase family protein -
M5T14_RS16940 (M5T14_16940) 3570313..3571200 - 888 WP_015571641.1 LysR family transcriptional regulator -
M5T14_RS16945 (M5T14_16945) 3571298..3571900 + 603 WP_017382759.1 short chain dehydrogenase -
M5T14_RS16950 (M5T14_16950) 3571897..3572628 - 732 WP_017382760.1 methyltransferase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - luxS 3564786..3618657 53871


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T256123 WP_015571638.1 NZ_CP103674:3568409-3568708 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT256123 WP_015571639.1 NZ_CP103674:3568719-3569084 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References