Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4882262..4882864 Replicon chromosome
Accession NZ_CP103673
Organism Escherichia coli strain 4069

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag M4U10_RS23235 Protein ID WP_000897305.1
Coordinates 4882553..4882864 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M4U10_RS23230 Protein ID WP_000356395.1
Coordinates 4882262..4882552 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4U10_RS23195 (4877886) 4877886..4878788 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
M4U10_RS23200 (4878785) 4878785..4879420 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
M4U10_RS23205 (4879417) 4879417..4880346 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
M4U10_RS23210 (4880528) 4880528..4880770 - 243 WP_001309881.1 CopG family transcriptional regulator -
M4U10_RS23215 (4880989) 4880989..4881207 - 219 WP_001295676.1 CopG family transcriptional regulator -
M4U10_RS23220 (4881626) 4881626..4881904 - 279 WP_001315112.1 hypothetical protein -
M4U10_RS23225 (4881956) 4881956..4882177 - 222 WP_001550354.1 hypothetical protein -
M4U10_RS23230 (4882262) 4882262..4882552 - 291 WP_000356395.1 NadS family protein Antitoxin
M4U10_RS23235 (4882553) 4882553..4882864 - 312 WP_000897305.1 hypothetical protein Toxin
M4U10_RS23240 (4883093) 4883093..4884001 + 909 WP_001550353.1 alpha/beta hydrolase -
M4U10_RS23245 (4884169) 4884169..4885083 - 915 WP_109553727.1 transposase -
M4U10_RS23250 (4885096) 4885096..4885983 - 888 Protein_4545 hypothetical protein -
M4U10_RS23255 (4886399) 4886399..4887340 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
M4U10_RS23260 (4887385) 4887385..4887822 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T256114 WP_000897305.1 NZ_CP103673:c4882864-4882553 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT256114 WP_000356395.1 NZ_CP103673:c4882552-4882262 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References