Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3767840..3768567 Replicon chromosome
Accession NZ_CP103673
Organism Escherichia coli strain 4069

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag M4U10_RS17890 Protein ID WP_000547555.1
Coordinates 3768256..3768567 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M4U10_RS17885 Protein ID WP_000126294.1
Coordinates 3767840..3768259 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4U10_RS17865 (3762925) 3762925..3763452 - 528 WP_001078777.1 electron transport protein HydN -
M4U10_RS17870 (3763601) 3763601..3764611 - 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
M4U10_RS17875 (3764871) 3764871..3766328 + 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
M4U10_RS17880 (3766337) 3766337..3767761 + 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
M4U10_RS17885 (3767840) 3767840..3768259 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
M4U10_RS17890 (3768256) 3768256..3768567 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
M4U10_RS17895 (3768734) 3768734..3769204 - 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
M4U10_RS17900 (3769197) 3769197..3769607 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
M4U10_RS17905 (3769604) 3769604..3770371 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
M4U10_RS17910 (3770371) 3770371..3770913 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
M4U10_RS17915 (3770923) 3770923..3772632 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T256107 WP_000547555.1 NZ_CP103673:c3768567-3768256 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT256107 WP_000126294.1 NZ_CP103673:c3768259-3767840 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References