Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 646769..647568 Replicon chromosome
Accession NZ_CP103673
Organism Escherichia coli strain 4069

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag M4U10_RS03125 Protein ID WP_000347270.1
Coordinates 646769..647233 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag M4U10_RS03130 Protein ID WP_001551693.1
Coordinates 647233..647568 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4U10_RS03095 (641770) 641770..642204 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M4U10_RS03100 (642222) 642222..643100 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M4U10_RS03105 (643090) 643090..643869 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M4U10_RS03110 (643880) 643880..644353 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M4U10_RS03115 (644376) 644376..645656 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
M4U10_RS03120 (645905) 645905..646714 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M4U10_RS03125 (646769) 646769..647233 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M4U10_RS03130 (647233) 647233..647568 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M4U10_RS03135 (647717) 647717..649288 - 1572 WP_001273738.1 galactarate dehydratase -
M4U10_RS03140 (649663) 649663..650997 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
M4U10_RS03145 (651013) 651013..651783 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T256093 WP_000347270.1 NZ_CP103673:c647233-646769 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT256093 WP_001551693.1 NZ_CP103673:c647568-647233 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References