Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1992411..1992913 Replicon chromosome
Accession NZ_CP103672
Organism Escherichia coli O25b:H4-ST131 strain 4433

Toxin (Protein)


Gene name yoeB Uniprot ID E2QNP2
Locus tag M5S92_RS09565 Protein ID WP_000767819.1
Coordinates 1992659..1992913 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID E2QNP3
Locus tag M5S92_RS09560 Protein ID WP_001259253.1
Coordinates 1992411..1992662 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S92_RS09535 (1987589) 1987589..1988656 - 1068 WP_000080057.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
M5S92_RS09540 (1988656) 1988656..1989726 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
M5S92_RS09545 (1989723) 1989723..1991027 - 1305 WP_001296211.1 histidinol dehydrogenase -
M5S92_RS09550 (1991033) 1991033..1991932 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
M5S92_RS09555 (1992078) 1992078..1992128 - 51 WP_001364200.1 his operon leader peptide -
M5S92_RS09560 (1992411) 1992411..1992662 + 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
M5S92_RS09565 (1992659) 1992659..1992913 + 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
M5S92_RS09570 (1992996) 1992996..1993820 + 825 WP_000754737.1 SDR family oxidoreductase -
M5S92_RS09575 (1993866) 1993866..1994795 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
M5S92_RS09580 (1995010) 1995010..1995072 + 63 WP_010723108.1 membrane protein YoeI -
M5S92_RS09585 (1995062) 1995062..1996420 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -
M5S92_RS09590 (1996637) 1996637..1997095 + 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1996637..1997095 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10110.53 Da        Isoelectric Point: 7.2749

>T256075 WP_000767819.1 NZ_CP103672:1992659-1992913 [Escherichia coli O25b:H4-ST131]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9365.62 Da        Isoelectric Point: 4.6127

>AT256075 WP_001259253.1 NZ_CP103672:1992411-1992662 [Escherichia coli O25b:H4-ST131]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E2QNP2

Antitoxin

Source ID Structure
AlphaFold DB E2QNP3

References