Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4881150..4881752 Replicon chromosome
Accession NZ_CP103657
Organism Escherichia coli strain 3150

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag M5R25_RS23600 Protein ID WP_000897305.1
Coordinates 4881441..4881752 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5R25_RS23595 Protein ID WP_000356397.1
Coordinates 4881150..4881440 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R25_RS23575 (4877652) 4877652..4878554 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
M5R25_RS23580 (4878551) 4878551..4879186 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
M5R25_RS23585 (4879183) 4879183..4880112 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
M5R25_RS23590 (4880327) 4880327..4880545 - 219 WP_001298592.1 CopG family transcriptional regulator -
M5R25_RS23595 (4881150) 4881150..4881440 - 291 WP_000356397.1 NadS family protein Antitoxin
M5R25_RS23600 (4881441) 4881441..4881752 - 312 WP_000897305.1 hypothetical protein Toxin
M5R25_RS23605 (4881981) 4881981..4882889 + 909 WP_001318161.1 alpha/beta hydrolase -
M5R25_RS23610 (4882953) 4882953..4883894 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
M5R25_RS23615 (4883939) 4883939..4884376 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
M5R25_RS23620 (4884373) 4884373..4885245 - 873 WP_000920762.1 virulence factor BrkB family protein -
M5R25_RS23625 (4885239) 4885239..4885838 - 600 WP_001314324.1 glucose-1-phosphatase -
M5R25_RS23630 (4885937) 4885937..4886722 - 786 WP_000059679.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T256061 WP_000897305.1 NZ_CP103657:c4881752-4881441 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT256061 WP_000356397.1 NZ_CP103657:c4881440-4881150 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References