Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3976273..3976967 Replicon chromosome
Accession NZ_CP103657
Organism Escherichia coli strain 3150

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag M5R25_RS19185 Protein ID WP_001263491.1
Coordinates 3976273..3976671 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag M5R25_RS19190 Protein ID WP_000554755.1
Coordinates 3976674..3976967 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R25_RS19155 (3971535) 3971535..3972992 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
M5R25_RS19160 (3973001) 3973001..3973282 + 282 WP_022645226.1 hypothetical protein -
M5R25_RS19165 (3973299) 3973299..3973808 - 510 WP_001361775.1 metal-dependent hydrolase -
M5R25_RS19170 (3973870) 3973870..3974484 - 615 WP_022645225.1 peptide chain release factor H -
M5R25_RS19175 (3974481) 3974481..3975620 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
M5R25_RS19180 (3975811) 3975811..3976263 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
M5R25_RS19185 (3976273) 3976273..3976671 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5R25_RS19190 (3976674) 3976674..3976967 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5R25_RS19195 (3977019) 3977019..3978074 - 1056 WP_022645222.1 DNA polymerase IV -
M5R25_RS19200 (3978145) 3978145..3978930 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
M5R25_RS19205 (3978902) 3978902..3980614 + 1713 Protein_3765 flagellar biosynthesis protein FlhA -
M5R25_RS19210 (3980712) 3980712..3981485 - 774 WP_022645219.1 C40 family peptidase -
M5R25_RS19215 (3981671) 3981671..3981931 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T256055 WP_001263491.1 NZ_CP103657:c3976671-3976273 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT256055 WP_000554755.1 NZ_CP103657:c3976967-3976674 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References