Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 652066..652865 Replicon chromosome
Accession NZ_CP103657
Organism Escherichia coli strain 3150

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag M5R25_RS03085 Protein ID WP_000347273.1
Coordinates 652066..652530 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag M5R25_RS03090 Protein ID WP_001308975.1
Coordinates 652530..652865 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R25_RS03055 (647067) 647067..647501 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5R25_RS03060 (647519) 647519..648397 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
M5R25_RS03065 (648387) 648387..649166 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5R25_RS03070 (649177) 649177..649650 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5R25_RS03075 (649673) 649673..650953 - 1281 WP_023908831.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5R25_RS03080 (651202) 651202..652011 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5R25_RS03085 (652066) 652066..652530 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5R25_RS03090 (652530) 652530..652865 - 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5R25_RS03095 (653014) 653014..654585 - 1572 WP_023908830.1 galactarate dehydratase -
M5R25_RS03100 (654960) 654960..656294 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
M5R25_RS03105 (656310) 656310..657080 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T256041 WP_000347273.1 NZ_CP103657:c652530-652066 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT256041 WP_001308975.1 NZ_CP103657:c652865-652530 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References