Detailed information of TA system TA025601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2085492..2085896
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ACIFE_RS16390
• Protein ID: WP_081463255.1
• Coordinates: 2085783..2085896 (-)
• Length: 38 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: ACIFE_RS10155
• Protein ID: WP_014029486.1
• Coordinates: 2085492..2085773 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS10135	2081201..2081653	-	453	Protein_2023	hypothetical protein	-
ACIFE_RS17230	2081540..2081788	+	249	WP_162470194.1	hypothetical protein	-
ACIFE_RS17235	2081837..2082013	-	177	WP_014029482.1	hypothetical protein	-
ACIFE_RS10140	2082035..2082391	-	357	WP_014029483.1	hypothetical protein	-
ACIFE_RS10145	2082373..2082660	-	288	WP_041651016.1	hypothetical protein	-
ACIFE_RS16385	2083200..2083382	-	183	WP_202796616.1	transposase	-
ACIFE_RS10150	2083451..2085016	-	1566	WP_049786851.1	chloride channel protein	-
ACIFE_RS10155	2085492..2085773	-	282	WP_014029486.1	HigA family addiction module antidote protein	Antitoxin
ACIFE_RS16390	2085783..2085896	-	114	WP_081463255.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACIFE_RS10160	2086046..2086735	-	690	WP_014029487.1	pirin-like bicupin family protein	-
ACIFE_RS10165	2086959..2087528	+	570	WP_143217870.1	lytic transglycosylase domain-containing protein	-
ACIFE_RS10170	2087552..2088076	-	525	WP_014029489.1	hypothetical protein	-
ACIFE_RS10175	2088261..2090666	-	2406	WP_014029490.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-37)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 38 a.a.
• Molecular weight: 4392.85 Da
• Isoelectric Point: 4.3200

>T25601 WP_081463255.1 NC_015942:c2085896-2085783 [Acidithiobacillus ferrivorans SS3]
MMGDLEGFWSVSVNGNWRLTFRFDGNHAELVDYLDYH

Nucleotide

• Length: 114 bp

>T25601 NC_015942:c2085896-2085783 [Acidithiobacillus ferrivorans SS3]
ATGATGGGTGACCTTGAGGGATTCTGGTCGGTGAGCGTGAATGGAAACTGGCGACTGACCTTTCGTTTCGACGGTAATCA
TGCAGAATTGGTTGATTATTTGGATTATCACTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10075.50 Da
• Isoelectric Point: 7.2030

>AT25601 WP_014029486.1 NC_015942:c2085773-2085492 [Acidithiobacillus ferrivorans SS3]
MSEMHNPAHPGEVLREYLPEGMSVTDAAKALNVTRQALSALLNGRSGVSAEMALRLEAALGVEAGFWLRTQVAYDLWEAR
QRGVARGVRNIAA

Nucleotide

• Length: 282 bp

>AT25601 NC_015942:c2085773-2085492 [Acidithiobacillus ferrivorans SS3]
ATGAGCGAAATGCACAATCCCGCACACCCCGGAGAGGTGTTGCGTGAATACTTGCCGGAGGGTATGAGTGTCACCGACGC
TGCAAAGGCGCTGAACGTGACTAGGCAGGCCTTGTCCGCGCTATTAAATGGACGGTCTGGCGTCAGTGCAGAGATGGCGC
TACGTCTCGAAGCAGCACTTGGTGTGGAAGCGGGGTTCTGGTTGCGAACGCAGGTGGCTTACGACCTCTGGGAGGCGCGG
CAGCGGGGAGTGGCTAGAGGCGTTCGAAACATCGCAGCCTGA