Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3817563..3818257 Replicon chromosome
Accession NZ_CP103645
Organism Escherichia coli strain 961

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M5R18_RS18510 Protein ID WP_001263489.1
Coordinates 3817563..3817961 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M5R18_RS18515 Protein ID WP_000554758.1
Coordinates 3817964..3818257 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3813151) 3813151..3813231 - 81 NuclAT_13 - -
- (3813151) 3813151..3813231 - 81 NuclAT_13 - -
- (3813151) 3813151..3813231 - 81 NuclAT_13 - -
- (3813151) 3813151..3813231 - 81 NuclAT_13 - -
M5R18_RS18485 (3813827) 3813827..3814285 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M5R18_RS18490 (3814546) 3814546..3816003 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M5R18_RS18495 (3816060) 3816060..3816581 - 522 Protein_3615 peptide chain release factor H -
M5R18_RS18500 (3816577) 3816577..3816783 - 207 Protein_3616 RtcB family protein -
M5R18_RS18505 (3817101) 3817101..3817553 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M5R18_RS18510 (3817563) 3817563..3817961 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5R18_RS18515 (3817964) 3817964..3818257 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5R18_RS18520 (3818309) 3818309..3819364 - 1056 WP_001226164.1 DNA polymerase IV -
M5R18_RS18525 (3819435) 3819435..3820220 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5R18_RS18530 (3820192) 3820192..3821904 + 1713 Protein_3622 flagellar biosynthesis protein FlhA -
M5R18_RS18535 (3822128) 3822128..3822625 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3817563..3832843 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T256009 WP_001263489.1 NZ_CP103645:c3817961-3817563 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT256009 WP_000554758.1 NZ_CP103645:c3818257-3817964 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References