Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1056020..1056747 Replicon chromosome
Accession NZ_CP103645
Organism Escherichia coli strain 961

Toxin (Protein)


Gene name higB Uniprot ID A0A829L2T9
Locus tag M5R18_RS05135 Protein ID WP_001521139.1
Coordinates 1056020..1056331 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5R18_RS05140 Protein ID WP_000126294.1
Coordinates 1056328..1056747 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R18_RS05110 (1051955) 1051955..1053664 + 1710 WP_001521140.1 formate hydrogenlyase subunit HycE -
M5R18_RS05115 (1053674) 1053674..1054216 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
M5R18_RS05120 (1054216) 1054216..1054983 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
M5R18_RS05125 (1054980) 1054980..1055390 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
M5R18_RS05130 (1055383) 1055383..1055853 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
M5R18_RS05135 (1056020) 1056020..1056331 + 312 WP_001521139.1 type II toxin-antitoxin system HigB family toxin Toxin
M5R18_RS05140 (1056328) 1056328..1056747 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
M5R18_RS05145 (1056826) 1056826..1058250 - 1425 WP_020234028.1 6-phospho-beta-glucosidase AscB -
M5R18_RS05150 (1058259) 1058259..1059716 - 1458 WP_020234027.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
M5R18_RS05155 (1059976) 1059976..1060986 + 1011 WP_015912547.1 DNA-binding transcriptional regulator AscG -
M5R18_RS05160 (1061135) 1061135..1061662 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12493.23 Da        Isoelectric Point: 9.4783

>T255997 WP_001521139.1 NZ_CP103645:1056020-1056331 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT255997 WP_000126294.1 NZ_CP103645:1056328-1056747 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L2T9

Antitoxin

Source ID Structure

References