Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3562316..3562991 Replicon chromosome
Accession NZ_CP103642
Organism Enterobacter hormaechei strain 4453

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag M5S55_RS17190 Protein ID WP_015571638.1
Coordinates 3562316..3562615 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag M5S55_RS17195 Protein ID WP_015571639.1
Coordinates 3562626..3562991 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S55_RS17180 (M5S55_17180) 3558693..3560885 + 2193 WP_017692801.1 type I secretion system permease/ATPase -
M5S55_RS17185 (M5S55_17185) 3560866..3562065 + 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
M5S55_RS17190 (M5S55_17190) 3562316..3562615 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5S55_RS17195 (M5S55_17195) 3562626..3562991 + 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
M5S55_RS17200 (M5S55_17200) 3563018..3564199 - 1182 WP_015571640.1 PLP-dependent aminotransferase family protein -
M5S55_RS17205 (M5S55_17205) 3564220..3565107 - 888 WP_015571641.1 LysR family transcriptional regulator -
M5S55_RS17210 (M5S55_17210) 3565205..3565807 + 603 WP_015571642.1 short chain dehydrogenase -
M5S55_RS17215 (M5S55_17215) 3565801..3566535 - 735 WP_017692800.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T255989 WP_015571638.1 NZ_CP103642:3562316-3562615 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT255989 WP_015571639.1 NZ_CP103642:3562626-3562991 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References