Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 36833..37563 Replicon chromosome
Accession NZ_CP103642
Organism Enterobacter hormaechei strain 4453

Toxin (Protein)


Gene name higB Uniprot ID A0A837FIL2
Locus tag M5S55_RS00170 Protein ID WP_023302825.1
Coordinates 36833..37147 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5S55_RS00175 Protein ID WP_023302826.1
Coordinates 37144..37563 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S55_RS00150 (M5S55_00150) 32499..33584 + 1086 Protein_29 cellulase family glycosylhydrolase -
M5S55_RS00155 (M5S55_00155) 33490..34674 - 1185 WP_015570118.1 multidrug efflux MFS transporter EmrD -
M5S55_RS00160 (M5S55_00160) 34850..35683 - 834 WP_015570117.1 DMT family transporter -
M5S55_RS00165 (M5S55_00165) 35746..36192 - 447 WP_003861064.1 GNAT family N-acetyltransferase -
M5S55_RS00170 (M5S55_00170) 36833..37147 + 315 WP_023302825.1 type II toxin-antitoxin system HigB family toxin Toxin
M5S55_RS00175 (M5S55_00175) 37144..37563 + 420 WP_023302826.1 helix-turn-helix domain-containing protein Antitoxin
M5S55_RS00180 (M5S55_00180) 37710..37808 + 99 WP_057979964.1 ilvB operon leader peptide IvbL -
M5S55_RS00185 (M5S55_00185) 37915..39603 + 1689 WP_023302827.1 acetolactate synthase large subunit -
M5S55_RS00190 (M5S55_00190) 39607..39894 + 288 WP_015570113.1 acetolactate synthase small subunit -
M5S55_RS00195 (M5S55_00195) 40020..40613 + 594 WP_003861054.1 transcriptional regulator UhpA -
M5S55_RS00200 (M5S55_00200) 40610..42115 + 1506 WP_017694010.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12415.51 Da        Isoelectric Point: 10.2825

>T255981 WP_023302825.1 NZ_CP103642:36833-37147 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15380.96 Da        Isoelectric Point: 5.2068

>AT255981 WP_023302826.1 NZ_CP103642:37144-37563 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FIL2

Antitoxin

Source ID Structure

References