Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1091956..1092683 Replicon chromosome
Accession NZ_CP103626
Organism Escherichia coli strain 4012

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag M5S78_RS05285 Protein ID WP_000547564.1
Coordinates 1091956..1092267 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5S78_RS05290 Protein ID WP_000126294.1
Coordinates 1092264..1092683 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S78_RS05255 (1087098) 1087098..1088807 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
M5S78_RS05260 (1088817) 1088817..1089359 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
M5S78_RS05265 (1089359) 1089359..1090126 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
M5S78_RS05270 (1090123) 1090123..1090533 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
M5S78_RS05275 (1090526) 1090526..1090996 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
M5S78_RS05280 (1091021) 1091021..1091782 + 762 WP_001026446.1 hypothetical protein -
M5S78_RS05285 (1091956) 1091956..1092267 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
M5S78_RS05290 (1092264) 1092264..1092683 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
M5S78_RS05295 (1092797) 1092797..1094221 - 1425 WP_000110313.1 6-phospho-beta-glucosidase AscB -
M5S78_RS05300 (1094230) 1094230..1095687 - 1458 WP_001107856.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
M5S78_RS05305 (1095947) 1095947..1096957 + 1011 WP_001363554.1 DNA-binding transcriptional regulator AscG -
M5S78_RS05310 (1097106) 1097106..1097633 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T255898 WP_000547564.1 NZ_CP103626:1091956-1092267 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT255898 WP_000126294.1 NZ_CP103626:1092264-1092683 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References