Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 603953..604752 Replicon chromosome
Accession NZ_CP103626
Organism Escherichia coli strain 4012

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag M5S78_RS02945 Protein ID WP_000347251.1
Coordinates 603953..604417 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5S78_RS02950 Protein ID WP_001307405.1
Coordinates 604417..604752 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S78_RS02915 (598954) 598954..599388 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5S78_RS02920 (599406) 599406..600284 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5S78_RS02925 (600274) 600274..601053 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5S78_RS02930 (601064) 601064..601537 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5S78_RS02935 (601560) 601560..602840 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5S78_RS02940 (603089) 603089..603898 + 810 WP_000072192.1 aga operon transcriptional regulator AgaR -
M5S78_RS02945 (603953) 603953..604417 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5S78_RS02950 (604417) 604417..604752 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5S78_RS02955 (604901) 604901..606472 - 1572 WP_001273761.1 galactarate dehydratase -
M5S78_RS02960 (606847) 606847..608181 + 1335 WP_000599653.1 galactarate/glucarate/glycerate transporter GarP -
M5S78_RS02965 (608197) 608197..608967 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T255894 WP_000347251.1 NZ_CP103626:c604417-603953 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT255894 WP_001307405.1 NZ_CP103626:c604752-604417 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References