Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4633128..4633730 Replicon chromosome
Accession NZ_CP103623
Organism Escherichia coli strain 3985

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag M5T36_RS22280 Protein ID WP_000897305.1
Coordinates 4633419..4633730 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5T36_RS22275 Protein ID WP_000356397.1
Coordinates 4633128..4633418 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T36_RS22250 (4629073) 4629073..4629975 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
M5T36_RS22255 (4629972) 4629972..4630607 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
M5T36_RS22260 (4630604) 4630604..4631533 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
M5T36_RS22265 (4631863) 4631863..4632105 - 243 WP_001086388.1 protein YiiF -
M5T36_RS22270 (4632324) 4632324..4632542 - 219 WP_001611167.1 CopG family transcriptional regulator -
M5T36_RS22275 (4633128) 4633128..4633418 - 291 WP_000356397.1 NadS family protein Antitoxin
M5T36_RS22280 (4633419) 4633419..4633730 - 312 WP_000897305.1 hypothetical protein Toxin
M5T36_RS22285 (4633959) 4633959..4634867 + 909 WP_001162704.1 alpha/beta hydrolase -
M5T36_RS22290 (4634931) 4634931..4635872 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
M5T36_RS22295 (4635917) 4635917..4636354 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
M5T36_RS22300 (4636351) 4636351..4637223 - 873 WP_000920762.1 virulence factor BrkB family protein -
M5T36_RS22305 (4637217) 4637217..4637816 - 600 WP_001295269.1 glucose-1-phosphatase -
M5T36_RS22310 (4637915) 4637915..4638700 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T255890 WP_000897305.1 NZ_CP103623:c4633730-4633419 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT255890 WP_000356397.1 NZ_CP103623:c4633418-4633128 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References