Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3827437..3828034 Replicon chromosome
Accession NZ_CP103621
Organism Klebsiella pneumoniae strain 433

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag M5S60_RS18735 Protein ID WP_004142563.1
Coordinates 3827717..3828034 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag M5S60_RS18730 Protein ID WP_004142561.1
Coordinates 3827437..3827724 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S60_RS18700 (3823517) 3823517..3823765 + 249 WP_002893037.1 DUF1158 domain-containing protein -
M5S60_RS18705 (3823783) 3823783..3824124 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
M5S60_RS18710 (3824155) 3824155..3825270 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
M5S60_RS18715 (3825450) 3825450..3826034 + 585 WP_002893026.1 TetR/AcrR family transcriptional regulator -
M5S60_RS18720 (3826031) 3826031..3826399 + 369 WP_002893024.1 MmcQ/YjbR family DNA-binding protein -
M5S60_RS18725 (3826519) 3826519..3827172 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
M5S60_RS18730 (3827437) 3827437..3827724 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
M5S60_RS18735 (3827717) 3827717..3828034 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5S60_RS18740 (3828219) 3828219..3829262 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
M5S60_RS18745 (3829929) 3829929..3830795 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
M5S60_RS18750 (3830904) 3830904..3832331 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T255865 WP_004142563.1 NZ_CP103621:c3828034-3827717 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT255865 WP_004142561.1 NZ_CP103621:c3827724-3827437 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References