Detailed information of TA system TA025586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 395430..396039
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G0JSP2
• Locus tag: ACIFE_RS01795
• Protein ID: WP_014027870.1
• Coordinates: 395430..395705 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: ACIFE_RS01800
• Protein ID: WP_014027871.1
• Coordinates: 395722..396039 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS01780	391400..391831	+	432	WP_014027867.1	hypothetical protein	-
ACIFE_RS01785	391828..394590	+	2763	WP_014027868.1	ParA family protein	-
ACIFE_RS01790	394604..395227	+	624	WP_014027869.1	RNA ligase family protein	-
ACIFE_RS01795	395430..395705	+	276	WP_014027870.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACIFE_RS01800	395722..396039	+	318	WP_014027871.1	HigA family addiction module antidote protein	Antitoxin
ACIFE_RS01805	396074..396856	+	783	WP_014027872.1	HNH endonuclease	-
ACIFE_RS01810	397834..398730	-	897	WP_014027874.1	DMT family transporter	-
ACIFE_RS16045	398723..399313	-	591	WP_014027875.1	DinB family protein	-
ACIFE_RS01815	399673..399882	-	210	WP_014027876.1	DUF4926 domain-containing protein	-
ACIFE_RS01820	399879..400292	-	414	WP_014027877.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	283765..484935	201170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10418.78 Da
• Isoelectric Point: 6.8605

>T25586 WP_014027870.1 NC_015942:395430-395705 [Acidithiobacillus ferrivorans SS3]
MIRSFVHKGLKQVFETGQSAKVGADLVTRCKRRLDAINAAVSPEELNVPGFDFHALEGEPKRYTVHVNGPYCITFEWRDG
DAWRIDLENYH

Nucleotide

• Length: 276 bp

>T25586 NC_015942:395430-395705 [Acidithiobacillus ferrivorans SS3]
TTGATACGAAGTTTTGTGCATAAGGGCCTCAAGCAGGTTTTCGAAACAGGTCAATCGGCGAAAGTCGGTGCGGATTTGGT
AACCCGCTGCAAGCGCAGGCTCGATGCCATAAACGCTGCGGTATCCCCGGAGGAGTTGAACGTGCCGGGTTTCGACTTCC
ATGCTTTGGAGGGGGAACCGAAGCGGTACACCGTTCATGTGAATGGACCCTATTGCATCACCTTCGAGTGGCGGGATGGG
GATGCCTGGCGAATTGATCTGGAGAATTACCATTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11745.58 Da
• Isoelectric Point: 6.7282

>AT25586 WP_014027871.1 NC_015942:395722-396039 [Acidithiobacillus ferrivorans SS3]
MNEMTVEQMDRIPTHPGEILREEVLPALDISVAGFARGLRVSRQTVHGILSERKGITPEMALRIGKYLGNGPGIWLRMQQ
AYDLTVAERRLAEELATIHKPEQAA

Nucleotide

• Length: 318 bp

>AT25586 NC_015942:395722-396039 [Acidithiobacillus ferrivorans SS3]
ATGAACGAAATGACCGTTGAGCAGATGGACCGGATACCGACGCATCCTGGCGAGATATTGCGGGAGGAGGTTCTGCCTGC
ACTGGATATCTCTGTAGCAGGGTTTGCCCGTGGCCTGCGGGTCTCGCGCCAGACCGTTCACGGCATCCTGTCTGAGCGCA
AGGGCATTACCCCAGAGATGGCCTTGCGTATCGGCAAGTATCTGGGTAACGGGCCGGGGATATGGTTACGCATGCAACAG
GCTTACGATTTGACCGTTGCGGAACGTCGGTTGGCCGAAGAACTGGCGACTATTCACAAGCCCGAGCAGGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1E7XRU5