Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3968077..3968674 Replicon chromosome
Accession NZ_CP103618
Organism Klebsiella pneumoniae strain 4383

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag M5T08_RS19350 Protein ID WP_004142563.1
Coordinates 3968357..3968674 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag M5T08_RS19345 Protein ID WP_004142561.1
Coordinates 3968077..3968364 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T08_RS19315 (3964157) 3964157..3964405 + 249 WP_020325243.1 DUF1158 domain-containing protein -
M5T08_RS19320 (3964423) 3964423..3964764 - 342 Protein_3790 RamA family antibiotic efflux transcriptional regulator -
M5T08_RS19325 (3964795) 3964795..3965910 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
M5T08_RS19330 (3966090) 3966090..3966671 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
M5T08_RS19335 (3966671) 3966671..3967039 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
M5T08_RS19340 (3967159) 3967159..3967812 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
M5T08_RS19345 (3968077) 3968077..3968364 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
M5T08_RS19350 (3968357) 3968357..3968674 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5T08_RS19355 (3968859) 3968859..3969902 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
M5T08_RS19360 (3970572) 3970572..3971438 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
M5T08_RS19365 (3971547) 3971547..3972974 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T255848 WP_004142563.1 NZ_CP103618:c3968674-3968357 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT255848 WP_004142561.1 NZ_CP103618:c3968364-3968077 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References