Detailed information of TA system TA025581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 1787418..1787930
• Replicon: chromosome
• Accession: NC_015875
• Organism: Streptococcus pseudopneumoniae IS7493

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: G0I700
• Locus tag: SPPN_RS09205
• Protein ID: WP_000924730.1
• Coordinates: 1787418..1787672 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: SPPN_RS09210
• Protein ID: WP_014017342.1
• Coordinates: 1787676..1787930 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPPN_RS09190	1782725..1783780	-	1056	WP_000248359.1	hypothetical protein	-
SPPN_RS11440	1783899..1785101	-	1203	WP_014017341.1	restriction endonuclease subunit S	-
SPPN_RS09200	1785094..1787202	-	2109	WP_000064455.1	type I restriction-modification system subunit M	-
SPPN_RS09205	1787418..1787672	-	255	WP_000924730.1	Txe/YoeB family addiction module toxin	Toxin
SPPN_RS09210	1787676..1787930	-	255	WP_014017342.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SPPN_RS09215	1788062..1789159	-	1098	WP_000587363.1	nucleotidyltransferase	-
SPPN_RS09220	1789169..1789915	-	747	WP_080564714.1	class I SAM-dependent methyltransferase	-
SPPN_RS09225	1790023..1790376	-	354	WP_001003025.1	ribosome silencing factor	-
SPPN_RS09230	1790377..1790970	-	594	WP_000121659.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SPPN_RS09235	1790970..1791599	-	630	WP_000151307.1	nicotinate-nucleotide adenylyltransferase	-
SPPN_RS09240	1791651..1791962	-	312	WP_000060163.1	ribosome assembly RNA-binding protein YhbY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10102.55 Da
• Isoelectric Point: 7.9716

>T25581 WP_000924730.1 NC_015875:c1787672-1787418 [Streptococcus pseudopneumoniae IS7493]
MLLKFTEDAWADYCYWQTQDKKTLKRINKLIKDIQRDPFTGIGKPEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSF
KDHY

Nucleotide

• Length: 255 bp

>T25581 NC_015875:c1787672-1787418 [Streptococcus pseudopneumoniae IS7493]
ATGCTGCTCAAGTTTACAGAAGATGCCTGGGCAGATTATTGCTACTGGCAAACTCAGGACAAGAAAACGTTAAAAAGAAT
CAATAAACTAATCAAGGATATTCAACGTGATCCCTTTACAGGAATAGGTAAGCCAGAACCACTCAAGTATGACTACCAAG
GAGCCTGGTCACGTCGTATCGACGCAGAAAATCGCTTGATTTATATGATGGATGGAGATAGCGTGGCTTTCTTGTCCTTT
AAAGATCATTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9748.04 Da
• Isoelectric Point: 4.8430

>AT25581 WP_014017342.1 NC_015875:c1787930-1787676 [Streptococcus pseudopneumoniae IS7493]
MEAVLYSTFRNHLKDYMKKVNDEFEPLTVINKNPDEDIVVLSRSEWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVH
VIEE

Nucleotide

• Length: 255 bp

>AT25581 NC_015875:c1787930-1787676 [Streptococcus pseudopneumoniae IS7493]
ATGGAAGCAGTTCTTTACTCAACATTCCGAAATCATTTAAAAGACTACATGAAGAAGGTCAATGATGAATTTGAGCCTTT
GACGGTGATCAATAAAAATCCAGACGAGGACATTGTAGTTCTTTCAAGGAGCGAATGGGACAGTATTCAGGAAACTTTGA
GAATCGCTCAAAATAAGGAGTTATCAGACAAGGTTTTGCGAGGAATGGCTCAAGTCCGTGCTGGAAGCACTCAAGTCCAT
GTTATTGAGGAGTGA

Structures

Toxin

• AlphaFold DB: G0I700