Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4312944..4313743 Replicon chromosome
Accession NZ_CP103609
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag M5S83_RS20770 Protein ID WP_000347267.1
Coordinates 4313279..4313743 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5S83_RS20765 Protein ID WP_001307405.1
Coordinates 4312944..4313279 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS20750 (4308730) 4308730..4309500 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
M5S83_RS20755 (4309516) 4309516..4310850 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
M5S83_RS20760 (4311225) 4311225..4312796 + 1572 WP_001273741.1 galactarate dehydratase -
M5S83_RS20765 (4312944) 4312944..4313279 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5S83_RS20770 (4313279) 4313279..4313743 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5S83_RS20775 (4313798) 4313798..4314607 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5S83_RS20780 (4314856) 4314856..4316136 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5S83_RS20785 (4316159) 4316159..4316632 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5S83_RS20790 (4316643) 4316643..4317422 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5S83_RS20795 (4317412) 4317412..4318290 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
M5S83_RS20800 (4318308) 4318308..4318742 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4302289..4313743 11454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T255808 WP_000347267.1 NZ_CP103609:4313279-4313743 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT255808 WP_001307405.1 NZ_CP103609:4312944-4313279 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References