Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1449907..1450586 Replicon chromosome
Accession NZ_CP103609
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name higB Uniprot ID U9XN95
Locus tag M5S83_RS07045 Protein ID WP_000057540.1
Coordinates 1449907..1450209 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag M5S83_RS07050 Protein ID WP_000806442.1
Coordinates 1450245..1450586 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS07025 (1445170) 1445170..1446390 - 1221 WP_001251608.1 fosmidomycin MFS transporter -
M5S83_RS07030 (1446608) 1446608..1448260 + 1653 WP_000771768.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
M5S83_RS07035 (1448297) 1448297..1448776 - 480 WP_000186631.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
M5S83_RS07040 (1448980) 1448980..1449774 - 795 WP_000365180.1 TraB/GumN family protein -
M5S83_RS07045 (1449907) 1449907..1450209 + 303 WP_000057540.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5S83_RS07050 (1450245) 1450245..1450586 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
M5S83_RS07055 (1450644) 1450644..1453148 - 2505 WP_023155691.1 copper-exporting P-type ATPase CopA -
M5S83_RS07060 (1453410) 1453410..1454342 + 933 WP_023155692.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-100)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11781.35 Da        Isoelectric Point: 10.2638

>T255797 WP_000057540.1 NZ_CP103609:1449907-1450209 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTSLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT255797 WP_000806442.1 NZ_CP103609:1450245-1450586 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XN95

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References