Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1155674..1156215 Replicon chromosome
Accession NZ_CP103609
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag M5S83_RS05560 Protein ID WP_000615976.1
Coordinates 1155674..1155952 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag M5S83_RS05565 Protein ID WP_000729704.1
Coordinates 1155955..1156215 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS05545 (1153256) 1153256..1153834 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
M5S83_RS05550 (1154040) 1154040..1154807 + 768 WP_000333380.1 class II glutamine amidotransferase -
M5S83_RS05555 (1154778) 1154778..1155518 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M5S83_RS05560 (1155674) 1155674..1155952 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M5S83_RS05565 (1155955) 1155955..1156215 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M5S83_RS05570 (1156401) 1156401..1157174 + 774 WP_000543899.1 C40 family peptidase -
M5S83_RS05575 (1157231) 1157231..1157587 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
M5S83_RS05580 (1157580) 1157580..1157858 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
M5S83_RS05585 (1157963) 1157963..1160056 - 2094 WP_023155854.1 flagellar biosynthesis protein FlhA -
M5S83_RS05590 (1160040) 1160040..1161179 - 1140 WP_023155855.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T255795 WP_000615976.1 NZ_CP103609:c1155952-1155674 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT255795 WP_000729704.1 NZ_CP103609:c1156215-1155955 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References