Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 281004..281606 Replicon chromosome
Accession NZ_CP103609
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag M5S83_RS01420 Protein ID WP_000897305.1
Coordinates 281004..281315 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5S83_RS01425 Protein ID WP_000356397.1
Coordinates 281316..281606 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS01390 (276034) 276034..276819 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
M5S83_RS01395 (276918) 276918..277517 + 600 WP_001295269.1 glucose-1-phosphatase -
M5S83_RS01400 (277511) 277511..278383 + 873 WP_000920762.1 virulence factor BrkB family protein -
M5S83_RS01405 (278380) 278380..278817 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
M5S83_RS01410 (278862) 278862..279803 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
M5S83_RS01415 (279867) 279867..280775 - 909 WP_001162704.1 alpha/beta hydrolase -
M5S83_RS01420 (281004) 281004..281315 + 312 WP_000897305.1 hypothetical protein Toxin
M5S83_RS01425 (281316) 281316..281606 + 291 WP_000356397.1 NadS family protein Antitoxin
M5S83_RS01430 (282192) 282192..282410 + 219 WP_001295676.1 CopG family transcriptional regulator -
M5S83_RS01435 (282629) 282629..282871 + 243 WP_001086388.1 protein YiiF -
M5S83_RS01440 (283201) 283201..284130 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
M5S83_RS01445 (284127) 284127..284762 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
M5S83_RS01450 (284759) 284759..285661 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T255791 WP_000897305.1 NZ_CP103609:281004-281315 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT255791 WP_000356397.1 NZ_CP103609:281316-281606 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References