Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3648446..3648987 Replicon chromosome
Accession NZ_CP103596
Organism Escherichia coli strain 4848

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag M5T19_RS17810 Protein ID WP_000615983.1
Coordinates 3648709..3648987 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag M5T19_RS17805 Protein ID WP_000729703.1
Coordinates 3648446..3648706 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T19_RS17785 (3644121) 3644121..3644906 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5T19_RS17790 (3644878) 3644878..3646590 + 1713 Protein_3478 flagellar biosynthesis protein FlhA -
M5T19_RS17795 (3646814) 3646814..3647311 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
M5T19_RS17800 (3647487) 3647487..3648236 - 750 WP_000056849.1 C40 family peptidase -
M5T19_RS17805 (3648446) 3648446..3648706 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M5T19_RS17810 (3648709) 3648709..3648987 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M5T19_RS17815 (3649143) 3649143..3649883 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M5T19_RS17820 (3649854) 3649854..3650621 - 768 WP_000333380.1 class II glutamine amidotransferase -
M5T19_RS17825 (3650827) 3650827..3651405 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3642249..3657529 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T255767 WP_000615983.1 NZ_CP103596:3648709-3648987 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT255767 WP_000729703.1 NZ_CP103596:3648446-3648706 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References