Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3642249..3642943 Replicon chromosome
Accession NZ_CP103596
Organism Escherichia coli strain 4848

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M5T19_RS17770 Protein ID WP_001263489.1
Coordinates 3642249..3642647 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M5T19_RS17775 Protein ID WP_000554758.1
Coordinates 3642650..3642943 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3637837) 3637837..3637917 - 81 NuclAT_11 - -
- (3637837) 3637837..3637917 - 81 NuclAT_11 - -
- (3637837) 3637837..3637917 - 81 NuclAT_11 - -
- (3637837) 3637837..3637917 - 81 NuclAT_11 - -
M5T19_RS17745 (3638513) 3638513..3638971 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M5T19_RS17750 (3639232) 3639232..3640689 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M5T19_RS17755 (3640746) 3640746..3641267 - 522 Protein_3471 peptide chain release factor H -
M5T19_RS17760 (3641263) 3641263..3641469 - 207 Protein_3472 RtcB family protein -
M5T19_RS17765 (3641787) 3641787..3642239 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M5T19_RS17770 (3642249) 3642249..3642647 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5T19_RS17775 (3642650) 3642650..3642943 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5T19_RS17780 (3642995) 3642995..3644050 - 1056 WP_001226164.1 DNA polymerase IV -
M5T19_RS17785 (3644121) 3644121..3644906 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M5T19_RS17790 (3644878) 3644878..3646590 + 1713 Protein_3478 flagellar biosynthesis protein FlhA -
M5T19_RS17795 (3646814) 3646814..3647311 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3642249..3657529 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T255766 WP_001263489.1 NZ_CP103596:c3642647-3642249 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT255766 WP_000554758.1 NZ_CP103596:c3642943-3642650 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References