Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3679519..3680198 Replicon chromosome
Accession NZ_CP103592
Organism Escherichia coli O25b:H4-ST131 strain 4224

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag M5S53_RS18250 Protein ID WP_000057523.1
Coordinates 3679896..3680198 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag M5S53_RS18245 Protein ID WP_000806442.1
Coordinates 3679519..3679860 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S53_RS18235 (3675763) 3675763..3676695 - 933 WP_000883041.1 glutaminase A -
M5S53_RS18240 (3676957) 3676957..3679461 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
M5S53_RS18245 (3679519) 3679519..3679860 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
M5S53_RS18250 (3679896) 3679896..3680198 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5S53_RS18255 (3680331) 3680331..3681125 + 795 WP_000365147.1 TraB/GumN family protein -
M5S53_RS18260 (3681329) 3681329..3681808 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
M5S53_RS18265 (3681832) 3681832..3682632 + 801 WP_000439798.1 hypothetical protein -
M5S53_RS18270 (3682629) 3682629..3683132 + 504 WP_000667000.1 hypothetical protein -
M5S53_RS18275 (3683170) 3683170..3684822 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T255734 WP_000057523.1 NZ_CP103592:c3680198-3679896 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT255734 WP_000806442.1 NZ_CP103592:c3679860-3679519 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References