Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 643205..643932 Replicon chromosome
Accession NZ_CP103592
Organism Escherichia coli O25b:H4-ST131 strain 4224

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag M5S53_RS03190 Protein ID WP_000550189.1
Coordinates 643205..643519 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M5S53_RS03195 Protein ID WP_000560269.1
Coordinates 643516..643932 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S53_RS03170 (639362) 639362..640348 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
M5S53_RS03175 (640427) 640427..641119 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
M5S53_RS03180 (641196) 641196..641699 - 504 WP_001295542.1 M48 family metallopeptidase -
M5S53_RS03185 (641784) 641784..642920 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
M5S53_RS03190 (643205) 643205..643519 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
M5S53_RS03195 (643516) 643516..643932 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
M5S53_RS03200 (643977) 643977..645995 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
M5S53_RS03205 (646221) 646221..648572 - 2352 WP_000695432.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T255724 WP_000550189.1 NZ_CP103592:643205-643519 [Escherichia coli O25b:H4-ST131]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT255724 WP_000560269.1 NZ_CP103592:643516-643932 [Escherichia coli O25b:H4-ST131]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References