Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 601425..602224 Replicon chromosome
Accession NZ_CP103592
Organism Escherichia coli O25b:H4-ST131 strain 4224

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag M5S53_RS02960 Protein ID WP_000347251.1
Coordinates 601425..601889 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag M5S53_RS02965 Protein ID WP_001296435.1
Coordinates 601889..602224 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S53_RS02930 (596426) 596426..596860 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5S53_RS02935 (596878) 596878..597756 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5S53_RS02940 (597746) 597746..598525 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5S53_RS02945 (598536) 598536..599009 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5S53_RS02950 (599032) 599032..600312 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5S53_RS02955 (600561) 600561..601370 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5S53_RS02960 (601425) 601425..601889 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5S53_RS02965 (601889) 601889..602224 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5S53_RS02970 (602373) 602373..603944 - 1572 WP_001273763.1 galactarate dehydratase -
M5S53_RS02975 (604319) 604319..605653 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
M5S53_RS02980 (605669) 605669..606439 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T255723 WP_000347251.1 NZ_CP103592:c601889-601425 [Escherichia coli O25b:H4-ST131]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT255723 WP_001296435.1 NZ_CP103592:c602224-601889 [Escherichia coli O25b:H4-ST131]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References