Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3947596..3948290 Replicon chromosome
Accession NZ_CP103589
Organism Escherichia coli strain 4750

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag M5T00_RS19190 Protein ID WP_001263500.1
Coordinates 3947596..3947994 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M5T00_RS19195 Protein ID WP_000554758.1
Coordinates 3947997..3948290 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T00_RS19165 (3942961) 3942961..3943419 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
M5T00_RS19170 (3943680) 3943680..3945137 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
M5T00_RS19175 (3945194) 3945194..3945808 - 615 WP_000602124.1 peptide chain release factor H -
M5T00_RS19180 (3945805) 3945805..3946944 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
M5T00_RS19185 (3947134) 3947134..3947586 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
M5T00_RS19190 (3947596) 3947596..3947994 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M5T00_RS19195 (3947997) 3947997..3948290 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M5T00_RS19200 (3948342) 3948342..3949397 - 1056 WP_001226168.1 DNA polymerase IV -
M5T00_RS19205 (3949468) 3949468..3950391 - 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
M5T00_RS19210 (3950394) 3950394..3951257 - 864 WP_001169518.1 flagellar motor stator protein MotA -
M5T00_RS19215 (3951270) 3951270..3951986 - 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
M5T00_RS19220 (3952006) 3952006..3952473 - 468 WP_000725261.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T255706 WP_001263500.1 NZ_CP103589:c3947994-3947596 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT255706 WP_000554758.1 NZ_CP103589:c3948290-3947997 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References