Detailed information of TA system TA025566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 18874..19715
• Replicon: chromosome
• Accession: NC_015856
• Organism: Collimonas fungivorans Ter331

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: G0A7T7
• Locus tag: CFU_RS00050
• Protein ID: WP_014004003.1
• Coordinates: 18874..19389 (-)
• Length: 172 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: CFU_RS00055
• Protein ID: WP_041740976.1
• Coordinates: 19386..19715 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFU_RS24245	14533..15315	+	783	WP_148264715.1	hypothetical protein	-
CFU_RS00040	15296..15883	+	588	WP_190275202.1	hypothetical protein	-
CFU_RS00045	16490..17983	+	1494	WP_014004002.1	RNA-directed DNA polymerase	-
CFU_RS23560	18188..18400	-	213	WP_081466375.1	helix-turn-helix domain-containing protein	-
CFU_RS23565	18612..18854	+	243	WP_190275203.1	SymE family type I addiction module toxin	-
CFU_RS00050	18874..19389	-	516	WP_014004003.1	type II toxin-antitoxin system YhaV family toxin	Toxin
CFU_RS00055	19386..19715	-	330	WP_041740976.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
CFU_RS00060	20194..21126	-	933	WP_014004005.1	AraC family transcriptional regulator	-
CFU_RS00065	21228..21971	+	744	WP_041740978.1	SDR family oxidoreductase	-
CFU_RS00070	22204..23436	-	1233	WP_041740979.1	Fic family protein	-
CFU_RS00075	23976..24575	-	600	WP_014004008.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (16-152)

Antitoxin

• PrlF antitoxin (8-107)

Sequences

Toxin

Protein

• Length: 172 a.a.
• Molecular weight: 19843.54 Da
• Isoelectric Point: 9.2094

>T25566 WP_014004003.1 NC_015856:c19389-18874 [Collimonas fungivorans Ter331]
MTGSKPAHLVVNGWTVFAHPLFLAQIETLMRQVEALKQKDPAEYTKKNPRRRLAAIAELAFEVIPQDPSRAEYRQGNTLG
EDHKHWFRAKFFQQYRLFFRYHAPSKVIVYAWVNDGGTMRAYESSDDAYRVFRKMLESGHPPDDWDQLLVEARSEGDRLQ
RIAAAEPLVQP

Nucleotide

• Length: 516 bp

>T25566 NC_015856:c19389-18874 [Collimonas fungivorans Ter331]
ATGACCGGCAGCAAACCTGCGCACCTGGTCGTCAACGGGTGGACAGTTTTCGCTCACCCGCTATTCCTTGCGCAAATTGA
AACGCTGATGCGGCAGGTCGAGGCACTCAAGCAAAAAGATCCCGCCGAATACACAAAAAAGAACCCCAGGAGGCGGCTGG
CAGCAATCGCCGAACTGGCGTTCGAGGTGATTCCGCAAGATCCGTCGCGGGCCGAATACCGGCAGGGAAACACCCTCGGC
GAAGACCACAAGCACTGGTTCCGAGCCAAGTTCTTCCAGCAGTACCGGCTGTTCTTCCGCTACCATGCACCAAGCAAAGT
GATCGTCTACGCGTGGGTGAACGACGGGGGCACCATGCGCGCCTATGAGAGCAGCGACGATGCCTACCGGGTGTTCCGCA
AGATGCTGGAGAGCGGCCATCCTCCGGACGACTGGGATCAACTATTGGTCGAGGCGCGTTCCGAAGGCGACCGTCTGCAG
CGTATCGCGGCAGCGGAACCACTGGTGCAGCCCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12062.54 Da
• Isoelectric Point: 4.3953

>AT25566 WP_041740976.1 NC_015856:c19715-19386 [Collimonas fungivorans Ter331]
MTASLEVESTLTDRYQTTVPETVRRALKLSKRDKIHYTIRPGGEVVLSRVEAAEEEDPVLGQFLGFLARDIASHPERLQA
VDANFVQRIQSLVGGVEVDLDTALPADDE

Nucleotide

• Length: 330 bp

>AT25566 NC_015856:c19715-19386 [Collimonas fungivorans Ter331]
ATGACTGCCTCCCTAGAAGTCGAATCGACCCTGACCGATCGCTACCAGACAACGGTGCCGGAAACCGTTCGCCGCGCCCT
GAAGCTGAGCAAGCGGGACAAGATTCACTACACGATCCGCCCCGGCGGTGAGGTCGTACTCTCCCGCGTGGAAGCGGCCG
AGGAAGAGGACCCGGTGCTCGGGCAGTTCCTGGGCTTTCTGGCCCGCGACATTGCCAGCCATCCGGAACGACTCCAAGCC
GTCGACGCCAATTTCGTGCAACGCATTCAGTCACTGGTCGGCGGTGTTGAAGTCGATCTGGATACCGCCTTGCCGGCAGA
TGATGAATGA

Structures

Toxin

• AlphaFold DB: G0A7T7